Journal: Smart Materials in Medicine

Article Title: Eggshell-derived amorphous calcium phosphate: Synthesis, characterization and bio-functions as bone graft materials in novel 3D osteoblastic spheroids model

doi: 10.1016/j.smaim.2023.04.001

Figure Lengend Snippet: Fig. 10. Confocal microscopic inspection of OPN and collagen I expression of MC-3T3-E1 cells in 3D spheroids with ACP particles embedded and 2D culture system, respectively. (A) Left panel: OPN and collagen I expression in different culture media in 3D reconstructed images; Right panel: maximal fluorescent projection of OPN and collagen I in 3D spheroids as shown in left penal; (B) OPN and collagen I expression in different culture media in a 2D culture system. Scale bar ¼ 100 μm.

Article Snippet: In brief, cells and spheroids were cultured for 7 days and then fixed with 4 wt % PFA for 20 min. After washing in PBS thrice, samples were treated with 1% (v/v) TritionX-100 (X100, Sigma-Aldrich, USA) for 20 min, followed by blocking in 1 wt % BSA/PBS solution for 1 h. Then, the samples were incubated in goat-anti-mouse OPN primary antibody (Ab) (AF808, R&D systems, USA) and rabbit-anti-mouse collagen I Ab (ab21286, Abcam, USA) at 4 C overnight.

Techniques: Expressing

Journal: CNS neuroscience & therapeutics

Article Title: Exploring the Synergistic Effects of Erinacines on Microglial Regulation and Alzheimer's Pathology Under Metabolic Stress.

doi: 10.1111/cns.70137

Figure Lengend Snippet: FIGURE 10 | Erinacines promote neurogenesis and secondary branching of immature neural dendrites in the hippocampus of HFSTZ-APP/PS1 mice. HFSTZ-APP/PS1 mice were fed with vehicle (Veh), HE-A (30 mg/kg), HE-C (30 mg/kg), or HE-S (30 mg/kg) (n = 6 for each group) and were injected with BrdU (50 mg/kg) for seven consecutive days before sacrifice, and brain sections were taken for tissue immunofluorescence staining. BrdU antibody was used to detect Type 2 neural progenitor (green), and Doublecortin (DCX) antibody was used to detect newborn neurons (red). Representative immunofluorescence staining of newborn neurons in the SGZ in the hippocampus (A). The arrows indicate BrdU+ Type2 neural progenitor, the open arrows indicate DCX+ new neurons, and the double arrows indicate BrdU+ DCX+ newborn neurons that have just completed differentiation. (B, C) Quantification of BrdU+ cells/mm (B) and DCX+ cells/mm (C) in the SGZ. The dendritic complexity was analyzed by laminar quantification (D). (E, F) Primary (pri.) and secondary (sec.) dendrites of the DCX+ cells are counted along the middle of GCL (green dashed line) and the outer edge of GCL (red dashed line), respectively. The sprouting ratio of the primary dendrites and the branching ratio of the DCX+ cells were shown. The results are presented as the mean ± SEM. Significant differences between the Veh group and the other groups are indicated by *p < 0.05, **p < 0.01, and ***p < 0.001. ML, molecular layer; GCL, granular cell layer; SGZ, subgranular zone.

Article Snippet: Primary antibodies include mouse anti- BrdU antibody (Santa Cruz, CA, USA, sc32,323) and rabbit anti- doublecortin (DCX) antibody (Abcam, ab18723, GR140153- 1).

Techniques: Injection, Immunofluorescence, Staining